Chapter 4 microbial cell disruption =============================================================================== Basic teaching content ----------------------------------- 4.1 Composition and structure of cell wall 4.2 common crushing methods 4.3 determination of crushing rate. Focus ----------------- 1. Calculation model of centrifugal sedimentation, physical concept of separation factor and application Difficulties ------------------------ 1. Structure and working principle of homogenizer. 2. Structure and working principle of ball mill The basic requirements of this chapter --------------------------------------------------- 1. Master the common cell crushing methods and the cell wall composition of gramnegative cells; 2. Master the principles and common equipment of biological, chemical, mechanical, and other methods Teaching video --------------------------------------------- .. toctree:: :maxdepth: 2 Video 1 Video 2 Video 3